From a faster molecular test of CoVID-19 to tools that could become antibody-producing antibodies in patients recovering from the disease, this year’s winning research community’s combined attention in a challenging year Show.
It is known that need is the mother of development. Indeed, 2020 has demonstrated to us that a worldwide pandemic is one genuine mother. Regularly, our Top 10 Innovations rivalry centers around lab advancements, apparatuses intended to plumb the secrets of fundamental science. Yet, as the researcher turned their sights to understanding SARS-CoV-2, the advancement scene changed as needs be, with new apparatuses created and existing advances twisted to address the pandemic. So this year at The Researcher, our yearly challenge consolidates innovations pointed toward comprehension and eventually taking care of the Coronavirus issue.
Among our free appointed authorities’ picks for 2020’s Top 10 Innovations in Health and Medical were center research facility advancements, for example, a solitary cell proteome analyzer and a desktop quality synthesizer—close by pandemic-centered items, including a fast Coronavirus test, an apparatus that can catch immune response profiles from the blood plasma of convalescing Covid patients, and a stage for portraying glycans in the spike protein that studs the outside of SARS-CoV-2. The opposition among heavenly entries was steep to the point that the current year’s Top 10 really contains 12 items, on account of two or three ties.
As trying as 2020 has been for us all, this turbulent year has brought forth encouraging items and approaches for clarifying the perplexing universe of science. What’s more, significantly more than that, 2020 has demonstrated that mainstream researchers, when confronted with a mutual issue, can meet people’s high expectations and meet up to pull together, research, and enhance. Here, The Researcher presents the apparatuses and advancements that make up the current year’s 2020 Top 10 Innovations in Medical Feild.
1. AbCellera Celium
In late March, the biotech firm Abcalera hosted a call with 40 researchers to review data collected on possible antibodies against SARS Covey 2. Using AbCellera’s advanced therapeutic microfluidics and single-cell analysis tools to examine samples from COVID-19 patients, the company’s team validated genetic sequences encoding hundreds of antibodies that could cure the disease.
It was heartbreaking to see all of this data by hand, however, the team fed it to Salem through the data, which adds up to more than a million pieces of data that are needed for these antibodies. Reveals which data can work better in patients. Possible therapy. In real-time, at the call to prayer, researchers used Salem to investigate these relationships and the LY-CoV555 antibody at home, which, months later, entered clinical trials as a possible CoVID-19 treatment, according to Maya Smith. That is to say, the creator of AbCellera and Salem. “I think that says it all.”
Before Salem hit the market in 2017, scientists brought back complex spreadsheets of data that worked together with Abcalera to find antibodies that were difficult to access, and it was hard to know. Where to go, says Smith. Using Salem, the data is presented in visual form and with the help of this tool “helps you to identify the right novel for your needs,” said Kodiak Sciences Protein in Paleo Alto, California. Engineer Fernando Correa told the scientist. It has identified antibodies for the treatment of retinal diseases in partnership with AbCellera and claims that the company’s microfluidics, single-cell analysis, and data visual acuity tool are “user-friendly” antibodies. Facilitates the discovery process. ”
2. Abbott ID NOW COVID-19 Test
Since 2014, Abbott’s ID NOW system has helped physicians detect influenza A and B, strep A, respiratory sensitivity virus (RSV), and more recently SARS-CoV-2 in less than 15 minutes. Provided. The toaster-sized device works by heating the nasal specimens in an acid solution that opens the envelope of the virus from the cracks and exposes their RNA, which is now detected by PCR machines. Rises at steady temperatures instead of the cooling cycle. The COVID-19 ID NOW test was the first test accessible to the American public after receiving emergency clearance from the U.S. Food and Drug Administration in late March.
Norman Moore, Abbott’s director of scientific affairs for infectious diseases, says the test is short-lived to prevent viral spread. “You’ll soon be suffering from the most contagious disease – and if we don’t get this fashionable result in time, what’s the point if a molecular test comes back in two weeks?” He tells the scientist.
With more than 23,000 identification devices in use, primarily in emergency care clinics and pharmacies in the United States, Moore says his team developed tests compatible with platforms for other infectious diseases such as sexually transmitted infections.
3. BioLegend TotalSeq
In 2017, researchers at the New York Genome Center published a new method called Seit-Seq that allows scientists to estimate proteins in individual cells when they replicate the same cell. Are CITE-Seq works by binding antibodies to oligonucleotides which can ultimately be formed to determine whether target proteins were present or joined to the same antibodies? BioScience, a life science company, is licensed by CITE-Seq and is a combination of 130 oligo-linked antibodies for mass screening of individual cell surface proteins for use in single-cell configuration, Total Sec. Developed by MC Human Universal Cocktail V1.0. The platform from 10X Genomics.
“There is no theoretical limit to how many proteins you can have now,” says Christopher “Kit” Nazar, head of biogen’s proteogenomics. He added that the company was already working to increase the number of antibodies involved in Kakil “which increases the chances of large-scale impartial discovery.”
“It’s groundbreaking in many ways,” says Eskemandra Clooney, an immunologist and pathologist at Harvard Medical School, and the Broad Institute at MIT and Harvard University, Massachusetts General Hospital. Like many researchers, Valani, one of the human cell atlas’ immune cell coordinators, studied Covid 19 this year. She has already used Bio Legend’s Cocktail, launched in early August at 5,350 for five single-use glasses, which analyzed blood samples from nearly 300 patients. Who tested positive for SARS-Co-2.
4. Seven Bridges GRAF
The release of the human reference genome in 2013 was a huge leap forward for biology, but as far as reality is concerned, it has fallen far short. Our genomes are of a variety that is not referenced in the genome, which was created by taking small samples from individuals, mainly of European descent. To calculate human genetic diversity, the bioinformatics firm Seven Bridges has developed a genomic analysis platform called GRAF that seeks to include every possible repetition of genetic sequences in any location.
The result is a graph of known variables at specific points in the genome, rather than a GRAF / PEN genome reference linear reference sequence. When genomes are linked to the GR reference, no omissions, additions, single nucleotide polymorphisms, or other variations are remembered because they may be linked to the linear reference genome.
With the goal of promoting the presence of prominent groups in genomic research, Seven Bridges announced in June that its GRAF Germaine variant would be free to access research workflows and GRAF / PEN genome references for scientific researchers. “This is the first production-grade workflow that incorporates ethnic information and human genome diversity to provide better call and alignment,” said Brandi Davis-Dusenberry, the company’s chief scientific officer.
“Hopefully, by calculating this complexity in the analysis, you’ll find the things you were missing out on,” says Bruce Gelb, of the Medicine Child Health and Development Institute at the Icahn School of Medicine in Mt. Director Bruce Gelb. Cena “It was an idea that has been floating around for years, but before Sean Bridge was doing it, no one had implemented the graph-based approach that is practical. They are the first to do so.”
Galeb is looking for variants of congenital heart defects using the GRAF platform and comparing the variants using traditional sequential analysis. So far, he says, the GRAF seems to be pointing out some variations that are being overlooked.
5. OXGENE TESSA
Ryan Cawood, CEO of a UK-based biotech company, says a major challenge in delivering gene therapy to patients’ cells is the cost of creating an adeno-associated virus (AAV), which is linked to genes. Interest is a common vector for interest. “The first AAV gene therapy product, which was approved in the European Union, costs 1 million,” he said. “If you wanted to treat a disease [targeting a large organ through a therapy] that you could use on thousands of people, you just couldn’t afford to make it work.”
Currently, Cawood says, the interstices of cultured human cells have been replaced with multiple plasmids to form AAV vectors containing selected genes. But plasma is expensive to make, and the transfer process is not very efficient. In contrast, infection with adenovirus naturally causes cells to naturally activate the replication of AAVs.
The problem is, adenoviruses also develop themselves and in turn contaminate AAV products. To address this issue, OXGNE developed a genetic switch that blocks the activity of an adenovirus within a cell halfway through its life cycle, so that the cell can transmit AV. Make a program to remove particles but not to create an adenovirus. When the virus goes away, you only get AAV. Count says you won’t find anything to return the adenovirus. He added that the company began selling its research-grade viral vector in September, which he called TESA, and added that it would begin offering clinical-grade content next year. The cost of a research-grade vector starts at 000 5,000 and depends on the size of the cells. We included it in 2020 Top 10 Innovations.
6. Codex DNA BioXp 3250 System
Biotech firm Codex DNA released the BIXEP-3250 system in August 2020 as a follow-up to BIXEP-3200 in 2014. An automated platform for on-demand DNA assembly and reproduction allows researchers to synthesize genes and genomes faster than ever before. Peter Duncan, director of product management at Codex DNA, says the vaccine has the potential to accelerate the development of diagnostics and treatment. This equipment can be used on cancer cells or a variety of infectious agents, including SARS-CO2.
Without BioXP-3250 or its predecessors, labs that want to synthesize DNA fragments, clones or the entire genome have to send samples to be processed by a third party. In addition to dealing with transit, such an operation can take weeks or months. With io 100,000 price Bixp 50 3250, up to 7,000 base pairs of DNA sequences can be collected at the push of a button.
Instead of coding the genetic script on the computer for specific experiments, customers can order a module that arrives in about two days, which is ready. The module contains a bar code that contains all the necessary information. When scanned by the device, instructions for the synthesis of the required DNA are uploaded. Duncan says a lab technician just needs to insert the module into the device and press Start.
“BioExp has enabled us to perform simple subclinical steps,” Mark Tornita, VP of Biotech Discovery at Toutic Biotherapy, told the scientist in an email about how the lab developed NGS libraries. Uses this tool to “All of these methods [that are being run] save us time and cost on BioXP.”
7. IsoPlexis Single-Cell Intracellular Proteome
ISO Plexis’s single-cell intracellular proteome solution has grown out of Celtic’s numerous labs to find better ways to monitor protein-protein interactions.
With the aim of developing targeted treatments, only two types of proteins can be detected in cancer cells at a given time, using traditional methods such as Western blot, mass spectrometry, and flu cytometry. With the Spolex system starting in July, researchers can monitor 30 or more protein pathways, with results available the same day.
With previous technology, phosphorylation was used to identify the function of individual proteins, without any insight into how they work together. The single-cell displays the intracellular proteome function in the same way but is also able to provide a perspective of the entire protein signaling pathways, revealing how the network works as a whole.
Understanding the entire network of cellular pathways allows researchers to better understand the effects of empty cell flow, says Sean McKay, CEO and co-founder of Isoloplace. In cancer, he added, this approach helps to evaluate the effectiveness of targeted therapies such as antibody therapy or small molecule drugs.
“Such pathways primarily explain how cells are activated, [which] is especially important for cancer, where phosphoprotein signaling is not only a sign of cancer,” says James Heath. That is to say, who created this technology eight years ago. But there is a huge focus on targeted blockers. It was on 7 in the list of 2020 Top 10 Innovations.
8. GigaGen Surge
Scientists have used intravenous immunoglobulin (IVIG) to treat immunodeficient or immunosuppressed patients and convalescent plasma to treat infectious diseases for more than a century. And one of the many treatments tested for COVID-19 is plasma. But biological samples from donors are not the most standard treatment. Enter Gigajon’s Surge platform, which uses single cells to “capture and rebuild” libraries of antibodies from blood donors. To build these libraries, the company runs donor blood samples through the Surge platform, which separates individual antibody-producing B cells into microdroplets and extracts RNA encoding antibodies. David Johnson, CEO of Gigajin, says the genetic sequence could “map out a person’s immune system.”
The company’s researchers then select some of these antibodies that could be engineered into mammalian cells to treat the regenerated antibodies, which they say in vitro experiments in animal models and Test-based, far more powerful than plasma or IVIG. Gigajin does not currently intend to sell the surplus but is using the platform to treat cancer, immunodeficiency disorders, and, more recently, Covid 19. Gigajin hopes that in early 2021, clinical trials will begin in the treatment of their COVID-19, which uses more than 12,500 antibodies from 16 donors. Johnson says, and then “eliminates the complexity of the immune system.” Antibody treatment that causes severe reactions.
Fred and Vicki Modal, who founded the Jeffrey Modal Foundation after the death of their son Jeffrey at the age of 15 due to early immunodeficiencies, say they are looking for alternatives to IVIG, which sometimes Occurs in very low supply and can lead to many side effects. Patient. “[Giga Gain] is giving the greatest gift of all. He is giving hope to [immunodeficient] patients,” says Fred Modal.
9. 10X Genomics Chromium Single Cell Multiome ATAC + Gene Expression
A few years ago, 10X Genomics launched the ATAC-Sec, an experiment to identify areas of open chromatin in a cell. The product ranks among the top 10 innovations of Scientist 2019. According to product marketing manager Laura de Murray, it wasn’t long before customers were making more and more claims, with her impressions: In September, 10X implemented the chromium single-cell ATAC + gene expression, which extracted both epigenetic and gene data from individual nuclei.
The platform tags mRNA and loose chromatin fragments from each nucleus with DNA barcodes, de Meyer explained, and then nucleic acids were expanded and analyzed. “You can actually start figuring out which regulatory elements in the genome activate or turn off the gene,” he says. It costs about $ 2400 per reaction for reagents and microfluidic chips.
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Ansu Stipati, an immunologist at Stanford University School of Medicine and former postdoctoral fellow at ATAC Sec co-developer Howard Chang, told scientists he was investigating the effects of epigenetic changes associated with T cell fatigue in biopsy specimens from cancer. Using a new test for Patient. When tired, T cells become less effective in fighting cancer, and “what we’re doing now with RNA and ATAC mechanisms is asking, each of them How does the molecular switch regulate the genes that cause this dysfunctional result in the cell? ” Says Satpati.
10.10X Genomics Visium Spatial Gene Expression Solution
Over the years, single-cell transchromatics has provided a wealth of information on gene expression for individual cells and cell types. Now, 10X Genomics has advanced the new technology of local transchromatics, which provides complete transchrome data for only one or a few cells, and at the same time shows what happens in the tissue sample where the gene is expressed.
Launched in October 2019, Visim Spatial Gene Expression Solution exposes mRNA-binding oligonucleotides to 55 micrometer areas at 5,000 locations within a tissue sample, and component expression data for histological images. Searches together. The technology was developed by the Swedish company Special Transchromics and was originally marketed by 10X Genomics in 2018. Then 10X further developed its product before launching Vizim last year.
The Vizim Spatial Gene Expression Solution, which sells for 1,000 per sample, has smaller and denser spots – and five times more – when the company inherited it, says Nakheel Rao. There are, local platforms. Its explanation increases. “We’ve also been able to select tens of thousands of unique molecular identifiers on this occasion, dramatically improving test sensitivity.”
Hope it was an informative article regarding the 2020 Top 10 Innovations in the medical field.